Molecular epidemiology of Cryptosporidium spp. in an agricultural area of northern Vietnam: A community survey

The purpose of this study was to investigate the occurrence Cryptosporidium infection and potential for transmission spp. between animals humans in northern Vietnam. A total 2715 samples (2120 human diarrheal samples, 471 non-diarrheal 124 animal stool samples) were collected through our community survey an agricultural area. All tested by direct immunofluorescence assay (DFA) using a fluorescent microscope. DNA extraction, PCR amplification three genes (COWP, SSU-rRNA, GP60), sequencing analysis performed identify Of 15 (10 2 3 positive COWP gene. Three species identified as C. canis (from six two one dog sample), hominis four samples), suis pig amplified and/or SSU-rRNA genes. In terms hominis, GP60 subtype IeA12G3T3 detected all samples. Although number very small, epidemiological data showed that emerging pattern each (C. canis, suis) different at site. While only respectively, from both dogs humans. We suspect infections site may be due environmental contamination with feces.